D0205
De Greef & Jacobs medium
Callus derived from leaf pieces of sugarbeet, exposed to a cold period of 3-9 weeks, could be regenerated into a normal plant after being returned to normal temperature. On media free of growthregulators, a regenerating callus could be -formed with a high regeneration capacity.
| Micro Elements | mg/l | µM |
|---|---|---|
| CoCl2.6H2O | 0.0025 | 0.01 |
| CuSO4.5H2O | 0.0025 | 0.01 |
| FeNaEDTA | 36.70 | 100.00 |
| H3BO3 | 10.62 | 171.76 |
| KI | 1.58 | 9.54 |
| MnSO4.H2O | 1.68 | 9.94 |
| Na2MoO4.2H2O | 0.0025 | 0.01 |
| ZnSO4.7H2O | 1.06 | 3.69 |
| Macro Elements | mg/l | mM |
|---|---|---|
| CaCl2 | 226.50 | 2.04 |
| KCl | 600.00 | 8.05 |
| KNO3 | 2000.00 | 19.78 |
| MgSO4 | 244.33 | 2.03 |
| NaH2PO4 | 250.00 | 2.08 |
| (NH4)2SO4 | 400.00 | 3.03 |
De Greef W. and Jacobs M. In vitro culture of the sugarbeet: Description of a cell line with high regeneration capacity., Plant Science Letters., 17, 55-61 (1979).
Hazard statements
- H315 Causes skin irritation
- H319 Causes serious eye irritation
- H335 May cause respiratory irritation
Precautionary statements
- P261 Avoid breathing dust/fume/gas/mist/vapours/spray
- P302 IF ON SKIN:
- P305 IF IN EYES:
- P351 Rinse continuously with water for several minutes
- P352 Wash with soap and water