E0208

Eriksson (ER) medium including vitamins

Eriksson medium was developed for cell suspension cultures of Haplopappus gracilis.

An increase in the growth of cell suspensions, especially with small inocula, was achieved by a 10% reduction of the concentration MS microelements, except for Fe and Zn. Equimolar replacement of ZnSO4.-7H2O by ZnNa2EDTA improved cell growth as well. MnEDTA and CoEDTA did not improve the growth of the cell culture. A reduction of Murashige and Skoog NH4NO3 concentration of 1650 to 1200 mg/l and an increase in phosphate to 2.5 mM also stimulated cell growth.

Micro Elements	mg/l	µM
CoCl₂.6H₂O	0.0025	0.01
CuSO₄.5H₂O	0.0025	0.01
FeNaEDTA	36.70	100.00
H₃BO₃	0.63	10.19
MnSO₄.H₂O	1.69	10.00
Na₂MoO₄.2H₂O	0.025	0.10
ZnSO₄.7H₂O	9.15	31.80

Macro Elements	mg/l	mM
CaCl₂	332.02	2.99
KH₂PO₄	340.00	2.50
KNO₃	1900.00	18.79
MgSO₄	180.54	1.50
NH₄NO₃	1200.00	14.99

Vitamins	mg/l	µM
Glycine	2.00	26.64
Nicotinic acid	0.50	4.06
Pyridoxine HCl	0.50	2.43
Thiamine HCl	0.50	1.48

Total concentration Micro and Macro elements including -vitamins: 4004.42 mg/l

Eriksson T., Physiol. Plant, 18, 976 (1965).

Hazard statements

H315 Causes skin irritation
H319 Causes serious eye irritation
H335 May cause respiratory irritation

Precautionary statements

P261 Avoid breathing dust/fume/gas/mist/vapours/spray
P302 IF ON SKIN:
P305 IF IN EYES:
P351 Rinse continuously with water for several minutes
P352 Wash with soap and water

Filename	Language	File size
E0208_GB.pdf	English	587 KB
E0208_NL.pdf	Nederlands	597 KB
E0208_DE.pdf	Deutsch	592 KB
E0208_FR.pdf	Français	617 KB
E0208_ES.pdf	Español	613 KB
E0208_IT.pdf	Italiano	607 KB
E0208_PL.pdf	Polish	623 KB
E0208_KO.pdf	Korean	724 KB
E0208_DA.pdf	Danish	583 KB
E0208_SV.pdf	Swedish	582 KB
E0208_FI.pdf	Finnish	579 KB
E0208_CS.pdf	Czech	609 KB
E0208_EL.pdf	Greek	642 KB
E0208_TR.pdf	Turkish	601 KB

Filename	Batch number	Expiry date	Language
E0208_P19363_GB.pdf	P19363	01-2025	English