Gamborg B5 medium including vitamins
B5 medium has been defined for the growth of cell suspensions of soybean root cells in the presence of 2,4 D. Nitrate was required in a concentration of 20-30 mM. An addition of 2 mM ammoniumsulphate led to an increase in cellgrowth. NH4+ when added as the sole source of nitrogen, did not support growth. Similar results were obtai-ned when NH4NO3 was substi-tuted for (NH4)2SO4. However, ammonium ions depressed growth when the concen-tration excee-ded 2 mM. Variati-ons in the concentrations of phosphate, calcium and magnesi-um resulted in relatively minor chan-ges in growth rate. Thiamine is known to be an essenti-al nutrient for cell growth and is increa-sed in concentrati-on up to 10 mg/l. B5 medium has been defined for the growth of cell suspensions of soybean root cells in the presence of 2,4 D. Nitrate was required in a concentration of 20-30 mM. An addition of 2 mM ammoniumsulphate led to an increase in cellgrowth. NH4+ when added as the sole source of nitrogen, did not support growth. Similar results were obtai-ned when NH4NO3 was substi-tuted for (NH4)2SO4. However, ammonium ions depressed growth when the concen-tration excee-ded 2 mM. Variati-ons in the concentrations of phosphate, calcium and magnesi-um resulted in relatively minor chan-ges in growth rate. Thiamine is known to be an essenti-al nutrient for cell growth and is increa-sed in concentrati-on up to 10 mg/l.
| Micro Elements | mg/l | µM |
|---|---|---|
| CoCl2.6H2O | 0.025 | 0.11 |
| CuSO4.5H2O | 0.025 | 0.10 |
| FeNaEDTA | 36.70 | 100.00 |
| H3BO3 | 3.00 | 48.52 |
| KI | 0.75 | 4.52 |
| MnSO4.H2O | 10.00 | 59.16 |
| Na2MoO4.2H2O | 0.25 | 1.03 |
| ZnSO4.7H2O | 2.00 | 6.96 |
| Macro Elements | mg/l | mM |
|---|---|---|
| CaCl2 | 113.23 | 1.02 |
| KNO3 | 2500.00 | 24.73 |
| MgSO4 | 121.56 | 1.01 |
| NaH2PO4 | 130.44 | 1.09 |
| (NH4)2SO4 | 134.00 | 1.01 |
| Vitamins | mg/l | µM |
|---|---|---|
| myo-Inositol | 100.00 | 554.94 |
| Nicotinic acid | 1.00 | 8.12 |
| Pyridoxine HCl | 1.00 | 4.86 |
| Thiamine HCl | 10.00 | 29.65 |
Total concentration Micro and Macro elements including -vitamins: 3163.98 mg/l
Gamborg O.L., Miller R.A., Ojima K., Nutrient requirement of suspensions cultures of soybean root cells. Exp. Cell Res., 50, 151 (1968).
Hazard statements
- H315 Causes skin irritation
- H319 Causes serious eye irritation
- H335 May cause respiratory irritation
Precautionary statements
- P261 Avoid breathing dust/fume/gas/mist/vapours/spray
- P302 IF ON SKIN:
- P305 IF IN EYES:
- P351 Rinse continuously with water for several minutes
- P352 Wash with soap and water
| Filename | Language | File size |
|---|---|---|
| G0210_GB.pdf | English | 504 KB |
| G0210_NL.pdf | Nederlands | 512 KB |
| G0210_DE.pdf | Deutsch | 508 KB |
| G0210_FR.pdf | Français | 532 KB |
| G0210_ES.pdf | Español | 529 KB |
| G0210_IT.pdf | Italiano | 523 KB |
| G0210_PL.pdf | Polish | 534 KB |
| G0210_KO.pdf | Korean | 643 KB |
| G0210_DA.pdf | Danish | 503 KB |
| G0210_SV.pdf | Swedish | 499 KB |
| G0210_FI.pdf | Finnish | 493 KB |
| G0210_CS.pdf | Czech | 526 KB |
| G0210_EL.pdf | Greek | 555 KB |
| G0210_TR.pdf | Turkish | 519 KB |