L0217

Litvay medium

Litvay’s medium is composed for the in vitro culture of cell suspension of Daucus carotus and finally for Pinus taeda L. An increase of Increasing the magnesium concentration from 0.75 mM to 7.5 mM and decreasing the calcium concentration from 1.5 mM to 0.15 mM was also of positive -influence. However, these alterations are not as drastical as the improvement by the enrichement of the medium by additio-nal -phosphate.the phosphate concentration from 0.5 mM to 2.5 mM was essential for improved cell growth and embryo-genesis.

Micro Elements	mg/l	µM
CoCl₂.6H₂O	0.125	0.53
CuSO₄.5H₂O	0.50	2.00
FeNaEDTA	36.70	100.00
H₃BO₃	31.00	501.37
KI	4.15	25.00
MnSO₄.H₂O	21.00	124.25
Na₂MoO₄.2H₂O	1.25	5.17
ZnSO₄.7H₂O	43.00	149.54

Macro Elements	mg/l	mM
CaCl₂	16.61	0.15
KH₂PO₄	340.00	2.50
KNO₃	1900.00	18.79
MgSO₄	903.38	7.51
NH₄NO₃	1650.00	20.61

Litvay J.D., Verma D.C., Morris A.J., Plant Cell Rep., 4, 325 (1985).

Hazard statements

H315 Causes skin irritation
H319 Causes serious eye irritation
H335 May cause respiratory irritation
H412 Harmful to aquatic life with long lasting effects

Precautionary statements

P261 Avoid breathing dust/fume/gas/mist/vapours/spray
P273 Avoid release to the environment
P302 IF ON SKIN:
P305 IF IN EYES:
P351 Rinse continuously with water for several minutes
P352 Wash with soap and water

Filename	Language	File size
L0217_GB.pdf	English	719 KB
L0217_NL.pdf	Nederlands	727 KB
L0217_DE.pdf	Deutsch	723 KB
L0217_FR.pdf	Français	751 KB
L0217_ES.pdf	Español	744 KB
L0217_IT.pdf	Italiano	736 KB
L0217_PL.pdf	Polish	749 KB
L0217_KO.pdf	Korean	859 KB
L0217_DA.pdf	Danish	717 KB
L0217_SV.pdf	Swedish	714 KB
L0217_FI.pdf	Finnish	708 KB
L0217_CS.pdf	Czech	739 KB
L0217_EL.pdf	Greek	773 KB
L0217_TR.pdf	Turkish	737 KB