Murashige & Skoog medium including B5 vitamins
MS medium is the most used tissue culture medium, of which many variations have been developed. The medium is originated from White’s medium and originally developed for the cultivation of Nicotiana tabacum calli. Compared to White medium, the concentration of all ingredients is increased. An increase to 50-60 mM nitrogen stimulated the growth of Nicotiana cells significantly, however a concentration of 80 mM and higher was clearly disadvantageous to the cells. The increase of all other elements, especially the macro -elements, also stimulated the growth of the calli. Due to the high concentration of minerals, MS medium is a very rich and -saline -medium and may be too salty to certain plant species. To avoid this problem, MS is often used with the micro elements in full concentration, but with the macro elements in respectively half or three-quarter of the concentration as originally described by the authors. Sometimes the original MS vitamines are replaced by the vitamins of Linsmaier and Gamborg B5 medium regarding the higher concentration of Thiamine in relation to the requirement of this vitamin by plants.
| Micro Elements | mg/l | µM |
|---|---|---|
| CoCl2.6H2O | 0.025 | 0.11 |
| CuSO4.5H2O | 0.025 | 0.10 |
| FeNaEDTA | 36.70 | 100.00 |
| H3BO3 | 6.20 | 100.27 |
| KI | 0.83 | 5.00 |
| MnSO4.H2O | 16.90 | 100.00 |
| Na2MoO4.2H2O | 0.25 | 1.03 |
| ZnSO4.7H2O | 8.60 | 29.91 |
| Macro Elements | mg/l | mM |
|---|---|---|
| CaCl2 | 332.02 | 2.99 |
| KH2PO4 | 170.00 | 1.25 |
| KNO3 | 1900.00 | 18.79 |
| MgSO4 | 180.54 | 1.50 |
| NH4NO3 | 1650.00 | 20.61 |
| Vitamins | mg/l | µM |
|---|---|---|
| myo-Inositol | 100.00 | 554.94 |
| Nicotinic acid | 1.00 | 8.12 |
| Pyridoxine HCl | 1.00 | 4.86 |
| Thiamine HCl | 10.00 | 29.65 |
Total concentration Micro and Macro elements including vitamins: 4414.09 mg/l
Murashige T. and Skoog F., Physiol. Plant, 15, 473 (1962).
Hazard statements
- H315 Causes skin irritation
- H319 Causes serious eye irritation
- H335 May cause respiratory irritation
Precautionary statements
- P261 Avoid breathing dust/fume/gas/mist/vapours/spray
- P302 IF ON SKIN:
- P305 IF IN EYES:
- P351 Rinse continuously with water for several minutes
- P352 Wash with soap and water
| Filename | Language | File size |
|---|---|---|
| M0231_GB.pdf | English | 595 KB |
| M0231_NL.pdf | Nederlands | 606 KB |
| M0231_DE.pdf | Deutsch | 600 KB |
| M0231_FR.pdf | Français | 628 KB |
| M0231_ES.pdf | Español | 623 KB |
| M0231_IT.pdf | Italiano | 619 KB |
| M0231_PL.pdf | Polish | 630 KB |
| M0231_KO.pdf | Korean | 739 KB |
| M0231_DA.pdf | Danish | 596 KB |
| M0231_SV.pdf | Swedish | 593 KB |
| M0231_FI.pdf | Finnish | 588 KB |
| M0231_CS.pdf | Czech | 622 KB |
| M0231_EL.pdf | Greek | 652 KB |
| M0231_TR.pdf | Turkish | 615 KB |