Murashige & Skoog medium mod.No. 3A
MS medium is the most used tissue culture medium, of which many variations have been developed. The medium is originated from White’s medium and originally developed for the cultivation of Nicotiana tabacum calli. Compared to White medium, the concentration of all ingredients is increased. An increase to 50-60 mM nitrogen stimulated the growth of Nicotiana cells significantly, however a concentration of 80 mM and higher was clearly disadvantageous to the cells. The increase of all other elements, especially the macro -elements, also stimulated the growth of the calli. Due to the high concentration of minerals, MS medium is a very rich and -saline -medium and may be too salty to certain plant species. To avoid this problem, MS is often used with the micro elements in full concentration, but with the macro elements in respectively half or three-quarter of the concentration as originally described by the authors. Sometimes the original MS vitamines are replaced by the vitamins of Linsmaier and Gamborg B5 medium regarding the higher concentration of Thiamine in relation to the requirement of this vitamin by plants.
| Micro Elements | mg/l | µM |
|---|---|---|
| CoCl2.6H2O | 0.025 | 0.11 |
| CuSO4.5H2O | 0.025 | 0.10 |
| FeNaEDTA | 36.70 | 100.00 |
| H3BO3 | 6.20 | 100.27 |
| KI | 0.83 | 5.00 |
| MnSO4.H2O | 16.90 | 100.00 |
| Na2MoO4.2H2O | 0.25 | 1.03 |
| ZnSO4.7H2O | 8.60 | 29.91 |
| Macro Elements | mg/l | mM |
|---|---|---|
| CaCl2 | 332.02 | 2.99 |
| KH2PO4 | 170.00 | 1.25 |
| KNO3 | 950.00 | 9.40 |
| MgSO4 | 180.54 | 1.50 |
| NH4NO3 | 825.00 | 10.30 |
Murashige T. and Skoog F., Physiol. Plant, 15, 473 (1962).
Hazard statements
- H315 Causes skin irritation
- H319 Causes serious eye irritation
- H335 May cause respiratory irritation
- H412 Harmful to aquatic life with long lasting effects
Precautionary statements
- P261 Avoid breathing dust/fume/gas/mist/vapours/spray
- P273 Avoid release to the environment
- P302 IF ON SKIN:
- P305 IF IN EYES:
- P351 Rinse continuously with water for several minutes
- P352 Wash with soap and water