M0243

Murashige & Miller medium stage I & II

Syngonium stage I & II

MS medium is the most used tissue culture medium, of which many variations have been developed. The medium is originated from White’s medium and originally developed for the cultivation of Nicotiana tabacum calli. Compared to White medium, the concentration of all ingredients is increased. An increase to 50-60 mM nitrogen stimulated the growth of Nicotiana cells significantly, however a concentration of 80 mM and higher was clearly disadvantageous to the cells. The increase of all other elements, especially the macro -elements, also stimulated the growth of the calli. Due to the high concentration of minerals, MS medium is a very rich and -saline -medium and may be too salty to certain plant species. To avoid this problem, MS is often used with the micro elements in full concentration, but with the macro elements in respectively half or three-quarter of the concentration as originally described by the authors. Sometimes the original MS vitamines are replaced by the vitamins of Linsmaier and Gamborg B5 medium regarding the higher concentration of Thiamine in relation to the requirement of this vitamin by plants.

Micro Elements mg/l µM
CoCl2.6H2O 0.025 0.11
CuSO4.5H2O 0.025 0.10
FeNaEDTA 36.70 100.00
H3BO3 6.20 100.27
KI 0.83 5.00
MnSO4.H2O 16.90 100.00
Na2MoO4.2H2O 0.25 1.03
ZnSO4.7H2O 8.60 29.91
Macro Elements mg/l mM
CaCl2 332.02 2.99
KH2PO4 170.00 1.25
KNO3 1900.00 18.79
MgSO4 180.54 1.50
NaH2PO4.anh 295.41 2.15
NH4NO3 1650.00 20.61
Vitamins mg/l µM
myo-Inositol 100.00 554.94
Thiamine HCl 0.40 0.30

Total concentration Micro and Macro elements including vitamins: 4697.90 mg/l

Murashige T. and Skoog F., Physiol. Plant, 15, 473 (1962).

Hazard statements

  • H315 Causes skin irritation
  • H319 Causes serious eye irritation
  • H335 May cause respiratory irritation

Precautionary statements

  • P261 Avoid breathing dust/fume/gas/mist/vapours/spray
  • P302 IF ON SKIN:
  • P305 IF IN EYES:
  • P351 Rinse continuously with water for several minutes
  • P352 Wash with soap and water
Filename Language File size
M0243_GB.pdf English 619 KB
M0243_NL.pdf Nederlands 632 KB
M0243_DE.pdf Deutsch 625 KB
M0243_FR.pdf Français 654 KB
M0243_ES.pdf Español 648 KB
M0243_IT.pdf Italiano 642 KB
M0243_PL.pdf Polish 654 KB
M0243_KO.pdf Korean 763 KB
M0243_DA.pdf Danish 620 KB
M0243_SV.pdf Swedish 617 KB
M0243_FI.pdf Finnish 613 KB
M0243_CS.pdf Czech 645 KB
M0243_EL.pdf Greek 677 KB
M0243_TR.pdf Turkish 638 KB
  • M0243.0010
  • 10 l
  • € 9.50
  • Order
  • M0243.0050
  • 50 l
  • € 29.50
  • Order
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