Quoirin & Lepoivre medium including vitamins
Prunus species plantlets could be regenerated from root callus on a medium defined by Quirin and Lepoivre. The calli were formed on the roots of plantlets derived from meristem culture containing 6-benzylaminopurine and Gibberellic acid. Micropropagation of Rosa hybrida L. cultivars is also described on this medium. Quoirin & Lepoivre medium has several differences in comparison to Murashige & Skoog. The ammonium ion concentration is strongly reduced, the calcium ion concentration is -increased and the chlorine ions are almost eliminated. This formulation avoids vitrification problems.
| Micro Elements | mg/l | µM |
|---|---|---|
| CoCl2.6H2O | 0.025 | |
| CuSO4.5H2O | 0.025 | |
| FeNaEDTA | 36.70 | |
| H3BO3 | 6.20 | |
| KI | 0.08 | |
| MnSO4.H2O | 0.76 | |
| Na2MoO4.2H2O | 0.25 | |
| ZnSO4.7H2O | 8.60 |
| Macro Elements | mg/l | mM |
|---|---|---|
| Ca(NO3)2.anhydrous | 578.92 | |
| KH2PO4 | 270.00 | |
| KNO3 | 1800.00 | |
| MgSO4 | 175.79 | |
| NH4NO3 | 400.00 |
| Vitamins | mg/l | µM |
|---|---|---|
| myo-Inositol | 100.00 | |
| Thiamine HCl | 0.40 |
Total concentration Micro and Macro elements including -vitamins: 3378.40 mg/l
Druart. P., Sci. Hort., 12, 339-342, (1980).,Quoirin M. and Lepoivre P., Acta Hort, 78, 437, (1977).,Valles. M., Boxus, Ph., Acta Hort., 212, (1987).
Hazard statements
- H315 Causes skin irritation
- H319 Causes serious eye irritation
- H335 May cause respiratory irritation
- H412 Harmful to aquatic life with long lasting effects
Precautionary statements
- P261 Avoid breathing dust/fume/gas/mist/vapours/spray
- P273 Avoid release to the environment
- P302 IF ON SKIN:
- P305 IF IN EYES:
- P351 Rinse continuously with water for several minutes
- P352 Wash with soap and water